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Irregular Expression of Cellular Stress Response Markers in the Placenta of Women with Chronic Venous Disease.
García-Montero, Cielo; Fraile-Martinez, Oscar; Rodriguez-Martín, Sonia; Funes Moñux, Rosa M; Saz, Jose V; Bravo, Coral; De Leon-Luis, Juan A; Ruiz-Minaya, María; Pekarek, Leonel; Saez, Miguel A; García-Lledo, Alberto; Alvarez-Mon, Melchor; Bujan, Julia; García-Honduvilla, Natalio; Ortega, Miguel A.
Affiliation
  • García-Montero C; Department of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcala de Henares, Spain.
  • Fraile-Martinez O; Ramón y Cajal Institute of Sanitary Research (IRYCIS), 28034 Madrid, Spain.
  • Rodriguez-Martín S; Department of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcala de Henares, Spain.
  • Funes Moñux RM; Ramón y Cajal Institute of Sanitary Research (IRYCIS), 28034 Madrid, Spain.
  • Saz JV; Department of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcala de Henares, Spain.
  • Bravo C; Service of Pediatric, Hospital Universitario Principe de Asturias, 28801 Alcala de Henares, Spain.
  • De Leon-Luis JA; Department of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcala de Henares, Spain.
  • Ruiz-Minaya M; Service of Pediatric, Hospital Universitario Principe de Asturias, 28801 Alcala de Henares, Spain.
  • Pekarek L; Department of Biomedicine and Biotechnology, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcala de Henares, Spain.
  • Saez MA; Department of Public and Maternal and Child Health, School of Medicine, Complutense University of Madrid, 28040 Madrid, Spain.
  • García-Lledo A; Department of Obstetrics and Gynecology, University Hospital Gregorio Marañón, 28009 Madrid, Spain.
  • Alvarez-Mon M; Health Research Institute Gregorio Marañón, 28009 Madrid, Spain.
  • Bujan J; Department of Public and Maternal and Child Health, School of Medicine, Complutense University of Madrid, 28040 Madrid, Spain.
  • García-Honduvilla N; Department of Obstetrics and Gynecology, University Hospital Gregorio Marañón, 28009 Madrid, Spain.
  • Ortega MA; Health Research Institute Gregorio Marañón, 28009 Madrid, Spain.
Antioxidants (Basel) ; 11(11)2022 Nov 17.
Article in En | MEDLINE | ID: mdl-36421463
Pregnancy comprises a period in a woman's life in which the circulatory system is subjected to hemodynamical and biochemical changes. During this period, while restructuring blood vessels and exchanging maternal-fetal products there is an increased risk of developing chronic venous disease (CVD), which may have an echo in life after childbirth for both mother and child. Previously, we investigated that pregnancy-associated CVD involves changes in placental architecture at angiogenesis, lymphangiogenesis and villi morphology compared with healthy controls (HC) with no history of CVD. We aimed to more deeply investigate the oxidative stress response in placenta from women with CVD versus HC through several markers (NRF2, KEAP1, CUL3, GSK-3ß). An observational, analytical, and prospective cohort study was conducted on 114 women in their third trimester of pregnancy (32 weeks). A total of 62 participants were clinically diagnosed with CVD. In parallel, 52 controls with no history of CVD (HC) were studied. Gene and protein expressions of NRF2, KEAP1, CUL3, GSK-3ß were analyzed by real-time polymerase chain reaction (RT-qPCR) and immunohistochemistry. Nrf2 gene and protein expression was significantly greater in placental villi of women with CVD, while Keap1, CUL-3 and GSK-3ß gene and protein expressions were significantly lower. Our results defined an aberrant gene and protein expression of Nrf2 and some of their main regulators Keap1, CUL-3 and GSK-3 ß in the placenta of women with CVD, which could be an indicator of an oxidative environment observed in this tissue.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Observational_studies / Risk_factors_studies Language: En Journal: Antioxidants (Basel) Year: 2022 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Observational_studies / Risk_factors_studies Language: En Journal: Antioxidants (Basel) Year: 2022 Document type: Article Affiliation country: Country of publication: